Isolation and characterization of Df(2L)BSC143. - Christensen, S.; Cook, K. (2006)
Isolation and characterization of Df(2L)BSC143 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC143 was isolated as a FLP recombinase-induced recombination event involving PBacWHCG5731[f00094] and PXPd05337a. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacWHCG5731[f00094]/PXPd05337a males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC143 from the segment of PBacWHCG5731[f00094] to the left of its FRT site and the segment of PXPd05337a to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC143 predicted from the transposable element insertions sites using Release 3 coordinates are 31B1;31D9. It failed to complement chico[1], trk[1], and bsk[1].