Isolation and characterization of Df(2R)BSC158. - Christensen, S.; Cook, K. (2006)
Isolation and characterization of Df(2R)BSC158 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC158 was isolated as a FLP recombinase-induced recombination event involving PXPCG12744[d01217] and PBacWHPka-R2[f07569]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPCG12744[d01217]/PBacWHPka-R2[f07569] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC158 from the segment of PXPCG12744[d01217] to the left of its FRT site and the segment of PBacWHPka-R2[f07569] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC158 predicted from the transposable element insertions sites using Release 4 coordinates are 46C1;46D1-4. It failed to complement Adam[5-3] and Mef2[X1].