Isolation and characterization of Df(3R)BSC196. - Gresens, J.; Cook, K. (2006)
Isolation and characterization of Df(3R)BSC196 Jill Gresens and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC196 was isolated as a FLP recombinase-induced recombination event involving PBacRBe01098 and PXPCG2747[d09536]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6B, Tb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacRBe01098/PXPCG2747[d09536] males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC196 from the segment of PBacRBe01098 to the left of its FRT site and the segment of PXPCG2747[d09536] to the right of its FRT site. The cytological breakpoints of Df(3R)BSC196 predicted from the transposable element insertions sites using Release 4 coordinates are 84E6;84E8. It failed to complement l(3)84Ed[1] and l(3)84Ec[1].