Isolation and characterization of Df(3R)BSC194. - Gresens, J.; Cook, K. (2006)
Isolation and characterization of Df(3R)BSC194 Jill Gresens and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC194 was isolated as a FLP recombinase-induced recombination event involving PBacWHOs-E[f00970] and PXPRm62[d09965]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6B, Tb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacWHOs-E[f00970]/PXPRm62[d09965] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC194 from the segment of PBacWHOs-E[f00970] to the left of its FRT site and the segment of PXPRm62[d09965] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC194 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 83D1;83D2. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail. It failed to complement Rm62[EY06795].