Isolation and characterization of Df(2L)BSC243. - Christensen, S.; Cook, K. (2006)
Isolation and characterization of Df(2L)BSC243 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC243 was isolated as a FLP recombinase-induced recombination event involving PBacWHf05591 and PXPd10374. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacWHf05591/ PXPd10374 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC243 from the segment of PBacWHf05591 to the left of its FRT site and the segment of PXPd10374 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd10374 to be at Release 3 genomic coordinate 12044040 on chromosome arm 2L. The Gene Disruption project determined the insertion site of PXPd10374 to be at Release 3 genomic coordinate 12044441 on arm 2L. This corresponds to 33C1 on both the Release 3 and Release 4 genome maps. The predicted position of PBacWHf05591 on the Release 4 map is 32F3. Consequently, the cytological breakpoints of Df(3R)BSC243 are predicted to be 32F3;33C1. It failed to complement esc[5].