Isolation and characterization of Df(3R)BSC221. - Gresens, J.; Cook, K. (2006)
Isolation and characterization of Df(3R)BSC221 Jill Gresens and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC221 was isolated as a FLP recombinase-induced recombination event involving PXPd11515 and PBacWHf00935. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPd11515/PBacWHf00935 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC221 from the segment of PXPd11515 to the left of its FRT site and the segment of PBacWHf00935 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd11515 to be at Release 3 genomic coordinate 2933219 on chromosome arm 3R. The Gene Disruption Project determined the insertion site of PXPd11515 to be at Release 3 genomic coordinate 2933501 on arm 3R. This corresponds to 84C1 on both the Release 3 and Release 4 genome maps. The predicted position of PBacWHf00935 on the Release 4 map is 84D2. Consequently, the cytological breakpoints of Df(3R)BSC221 are predicted to be 84C1;84D2. Df(3R)BSC221 failed to complement lap[1], sas[15] and l(3)s2214[s2214].