Isolation and characterization of Df(1)BSC285. - Christensen, S.; Cook, K. (2007)
Isolation and characterization of Df(1)BSC285 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(1)285 was isolated as a FLP recombinase-induced recombination event involving PBacWHCG14439[f06522] and PXPd08728. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118] PBacWHCG14439[f06522]/w[1118] PXPd08728; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC285 from the segment of PBacWHCG14439[f06522] to the left of its FRT site and the segment of PXPd08728 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)285 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 6C11;6D3. It failed to complement shf[2].