Isolation and characterization of Df(2L)BSC293. - Christensen, S.; Cook, K. (2007)
Isolation and characterization of Df(2L)BSC293 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC293 was isolated as a FLP recombinase-induced recombination event involving PXPd07167 and PBacWHPRL-1[f06562]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd07167/PBacWHPRL-1[f06562] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC293 from the segment of PXPd07167 to the left of its FRT site and the segment of PBacWHPRL-1[f06562] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC293 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 35E1;35F1. It failed to complement BicC[4].