Isolation and characterization of Df(2L)BSC203. - Christensen, S.; Cook, K. (2006)
Isolation and characterization of Df(2L)BSC203 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC203 was isolated as a FLP recombinase-induced recombination event involving PXPd03596 and PBacWHCG18661[f04576]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd03596/PBacWHCG18661[f04576] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC203 from the segment of PXPd03596 to the left of its FRT site and the segment of PBacWHCG18661[f04576] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC203 predicted from the transposable element insertions sites using Release 4 are 29D5;29F7. It failed to complement raw[1].