Isolation and characterization of Df(2L)BSC200. - Christensen, S.; Cook, K. (2006)
Isolation and characterization of Df(2L)BSC200 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC200 was isolated as a FLP recombinase-induced recombination event involving PBacWHCG8086[f03214] and PXPd05178. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacWHCG8086[f03214]/PXPd05178 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC200 from the segment of PBacWHCG8086[f03214] to the left of its FRT site and the segment of PXPd05178 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC200 predicted from the transposable element insertions sites using Release 4 coordinates are 28F5;29B1. It failed to complement Btk29A[k00206] and Df(2L)BSC111.