Isolation and characterization of Df(3L)BSC224. - Gresens, J.; Cook, K. (2006)
Isolation and characterization of Df(3L)BSC224 Jill Gresens and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC224 was isolated as a FLP recombinase-induced recombination event involving PXPsgl[d09725] and PBacWHcorn[f06151]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPsgl[d09725]/PBacWHcorn[f06151] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC224 from the segment of PXPsgl[d09725] to the left of its FRT site and the segment of PBacWHcorn[f06151] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC224 predicted from the Release 4 genomic coordinates of the transposable element insertions sites using are 65D5;65E6. Df(3L)BSC224 failed to complement bin[I1].