Isolation and characterization of Df(2L)BSC278. - Christensen, S.; Cook, K. (2007)
Isolation and characterization of Df(2L)BSC278 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC278 was isolated as a FLP recombinase-induced recombination event involving PXPd07167 and PBacRBpkaap[e04176]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd07167/PBacRBpkaap[e04176] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC278 from the segment of PXPd07167 to the left of its FRT site and the segment of PBacRBpkaap[e04176] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2L)BSC278 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 35E1;35F1. It failed to complement BicC[4] and crp[k00809].