Isolation and characterization of Df(1)BSC288. - Christensen, S.; Cook, K. (2007)
Isolation and characterization of Df(1)BSC288 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC288 was isolated as a FLP recombinase-induced recombination event involving PBacWHf02333 and PXPd05722. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118] PBacWHf02333/w[1118] PXPd05722; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC288 from the segment of PBacWHf02333 to the left of its FRT site and the segment of PXPd05722 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC288 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 10B2;10B11. It failed to complement dsh[1].