Isolation and characterization of Df(2L)BSC277. - Christensen, S.; Cook, K. (2007)
Isolation and characterization of Df(2L)BSC277 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC277 was isolated as a FLP recombinase-induced recombination event involving PXPd05987 and PBacWHCG31851[f03572]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd05987/PBacWHCG31851[f03572] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC277 from the segment of PXPd05987 to the left of its FRT site and the segment of PBacWHCG31851[f03572] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd05987 to be at Release 3 genomic coordinate 12821657 on chromosome arm 2L. The Gene Disruption project determined the insertion site of PXPd05987 to be at Release 3 genomic coordinate 12821673 on arm 2L. This corresponds to 34A1 on the Release 5 genome map. The predicted position of PBacWHCG31851[f03572] on the Release 5 map is 34B2. Consequently, the cytological breakpoints of Df(2L)BSC277 are predicted to be 34A1;34B2. It failed to complement Vha68-2[s4214] and Df(2L)Exel7055.