Isolation and characterization of Df(3R)BSC316. - Christensen, S.; Gresens, J.; Cook, K.; Cook, K. (2007)
Isolation and characterization of Df(3R)BSC316 Stacey Christensen, Jill Gresens, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC316 was isolated as a FLP recombinase-induced recombination event involving PBacRBtub[e03259] and PXPd09816. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6B females crossed to P{hsFLP}1, w[1118]; PBacRBtub[e03259]/PXPd09816 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC316 from the segment of PBacRBtub[e03259] to the left of its FRT site and the segment of PXPd09816 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(3R)BSC316 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 82A5;82B3. Df(3R)FDD-0201678 is a synonym for Df(3R)BSC316.