Isolation and characterization of Df(3L)BSC386. - Christensen, S.; Cook, K.; Cook, K. (2007)
Isolation and characterization of Df(3L)BSC386 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC386 was isolated as a FLP recombinase-induced recombination event involving PXPSk2[d05405] and PBacRBCG32280[e03473]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPSk2[d05405]/PBacRBCG32280[e03473] males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC386 from the segment of PXPSk2[d05405] to the left of its FRT site and the segment of PBacRBCG32280[e03473] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(3L)BSC386 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 63A3;63B8. Df(3L)BSC386 failed to complement rasp[m47]. Df(3L)FDD-0069201 is a synonym for Df(3L)BSC386.