Isolation and characterization of Df(3L)BSC448. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(3L)BSC448 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC448 was isolated as a FLP recombinase-induced recombination event involving PBacWHCG32425[f01627] and PXPd06749. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacWHCG32425[f01627]/PXPd06749 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC448 from the segment of PBacWHCG32425[f01627] to the left of its FRT site and the segment of PXPd06749 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC448 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 77C6;77E4. Df(3L)BSC448 failed to complement kni[ri-1].