Isolation and characterization of Df(3R)BSC490. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(3R)BSC490 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC490 was isolated as a FLP recombinase-induced recombination event involving PXPd03365 and PBacWHCG10217[f03018]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPd03365/PBacWHCG10217[f03018] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC490 from the segment of PXPd03365 to the left of its FRT site and the segment of PBacWHCG10217[f03018] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC490 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 95A7;95B1. Df(3R)BSC490 failed to complement Hmgcr[01152] and Tbp-1[04210b].