Isolation and characterization of Df(2L)BSC242. - Christensen, S.; Cook, K. (2008)
Isolation and characterization of Df(2L)BSC242 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC242 was isolated as a FLP recombinase-induced recombination event involving PXPd07625 and PBacWHf02178. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd07625/PBacWHf02178 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC242 from the segment of PXPd07625 to the left of its FRT site and the segment of PBacWHf02178 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC242 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 32D4;32F4. It failed to complement ab[1], cmet[04431] and salm[1].