Isolation and characterization of Df(3R)BSC523. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(3R)BSC523 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC523 was isolated as a FLP recombinase-induced recombination event involving PXPd07598 and PBacWHCG34376[f05438]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPd07598/PBacWHCG34376[f05438] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC523 from the segment of PXPd07598 to the left of its FRT site and the segment of PBacWHCG34376[f05438] to the right of its FRT site. The cytological breakpoints of Df(3R)BSC523 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 93F14;94A14. Df(3R)BSC523 failed to complement how[stru-3R-3] and epsin-like[03685].