Isolation and characterization of Df(3R)BSC567. - Cook, K.; Christensen, S.; Cook, K. (2008)
Isolation and characterization of Df(3R)BSC567 Kim Cook, Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC567 was isolated as a FLP recombinase-induced recombination event involving PXPMes-4[d03569] and PBacWHf06938. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPMes-4[d03569]/PBacWHf06938 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC567 from the segment of PXPMes-4[d03569] to the left of its FRT site and the segment of PBacWHf06938 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC567 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 98B6;98E55. Df(3R)BSC567 failed to complement fkh[6].