Isolation and characterization of Df(1)BSC571. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(1)BSC571 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC571 was isolated as a FLP recombinase-induced recombination event involving PBacWHCG33980[f02921] and PXPd04844. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBacWHCG33980[f02921]/PXPd04844; M KRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC571 from the segment of PBacWHCG33980[f02921] to the left of its FRT site and the segment of PXPd04844 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC571 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 5A4;5A10. It was viable and fertile in combination with Dp(1;Y)dx[+]5, but failed to complement l(1)G0045[G0312].