Isolation and characterization of Df(2R)BSC266. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(2R)BSC266 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC266 was isolated as a FLP recombinase-induced recombination event involving PBacWHHey[f06656] and PXPd08527. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacWHHey[f06656]/PXPd08527 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC266 from the segment of PBacWHHey[f06656] to the left of its FRT site and the segment of PXPd08527 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd08527 to be Release 3 genomic coordinate 3523385 on chromosome arm 2R. This corresponds to 44D1 on the Release 3 and Release 5 genome maps. The predicted position of PBacWHHey[f06656] on the Release 5 map is 44A2. Consequently, the cytological breakpoints of Df(2R)BSC266 are predicted to be 44A2;44D1. It failed to complement cul-4[KG02900], Pabp2[KG02359] and pnut[XP].