Isolation and characterization of Df(2R)BSC695. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(2R)BSC695 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC695 was isolated as a FLP recombinase-induced recombination event involving PBacWHCG30184[f04532] and PXPd11424. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacWHCG30184[f04532]/PXPd11424 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC695 from the segment of PBacWHCG30184[f04532] to the left of its FRT site and the segment of PXPd11424 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd11424 to be Release 3 genomic coordinate 19035686 on chromosome arm 2R. The Gene Disruption Project determined the insertion site of PXPd11424 to be Release 3 coordinate 19035692 on chromosome arm 2R. This corresponds to 60B1 on the Release 5 genome map. The predicted position of PBacWHCG30184[f04532] on the Release 5 map is 59F1. Consequently, the cytological breakpoints of Df(3R)BSC695 are predicted to be 59F1;60B1. Df(2R)BSC695 failed to complement retn[02535], or[49h] and bgcn[1].