Isolation and characterization of Df(1)BSC717. - Christensen, S.; Cook, K.; Cook, K. (2008)
Isolation and characterization of Df(1)BSC717 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC717 was isolated as a FLP recombinase-induced recombination event involving PBacRBCG3191[e02435] and PXPd01530. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of PBacRBCG3191[e02435] w[1118]/PXPd01530 w[1118]; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP3.RB5BSC717 from the segment of PBacRBCG3191[e02435] to the left of its FRT site and the segment of PXPd01530 to the right of its FRT site. The cytological breakpoints of Df(1)717 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 2F2;3A4. It failed to complement gt[1] and phl[11].