Isolation and characterization of Df(3R)BSC751. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(3R)BSC751 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC751 was isolated as a FLP recombinase-induced recombination event involving PBacWHf01987 and PXPd07975. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacWHf01987/PXPd07975 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC751 from the segment of PBacWHf01987 to the left of its FRT site and the segment of PXPd07975 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd07975 to be Release 3 genomic coordinate 21870411 on chromosome arm 3R. This corresponds to 96F10 on the Release 3 and Release 5 genome maps. The predicted position of PBacWHf01987 on the Release 5 map is 96F8. Consequently, the cytological breakpoints of Df(3R)BSC751 are predicted to be 96F8;96F10. Df(3R)BSC751 failed to complement gro[1].