Isolation and characterization of Df(3R)BSC746. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(3R)BSC746 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC746 was isolated as a FLP recombinase-induced recombination event involving PBacRBe00916 and PXPd03025. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacRBe00916/PXPd03025 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC746 from the segment of PBacRBe00916 to the left of its FRT site and the segment of PXPd03025 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. Exelixis, Inc. determined the insertion site of PXPd03025 to be Release 3 genomic coordinate 2231048 on chromosome arm 3R. This corresponds to 83E6 on the Release 3 and Release 5 genome maps. The predicted position of PBacRBe00916 on the Release 5 map is 83E2. Consequently, the cytological breakpoints of Df(3R)BSC746 are predicted to be 83E2;83E6. Df(3R)BSC746 failed to complement Df(3R)BSC681 and Df(3R)WIN11.