Isolation and characterization of Df(3L)BSC734. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(3L)BSC734 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC734 was isolated as a FLP recombinase-induced recombination event involving PBacWHf04670 and PXPtrbl[d03196]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacWHf04670/PXPtrbl[d03196] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC734 from the segment of PBacWHf04670 to the left of its FRT site and the segment of PXPtrbl[d03196] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC734 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 77A4;77C1. Df(3L)BSC734 failed to complement polo[16-1] and fbl[1].