Isolation and characterization of Df(2R)BSC769. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(2R)BSC769 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC769 was isolated as a FLP recombinase-induced recombination event involving PXPd01325 and PBacRBCG30414[e03801]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd01325/PBacRBCG30414[e03801] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC769 from the segment of PXPd01325 to the left of its FRT site and the segment of PBacRBCG30414[e03801] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2R)BSC769 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 59B7;59D9. Df(2R)BSC769 failed to complement twi[1] and l(2)06496[06496].