Isolation and characterization of Df(3L)BSC774. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(3L)BSC774 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC774 was isolated as a FLP recombinase-induced recombination event involving PXPd05671 and PBacWHf06011. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPd05671/PBacWHf06011 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC774 from the segment of PXPd05671 to the left of its FRT site and the segment of PBacWHf06011 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC774 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:15693003;16233373..16233380 and the cytological breakpoints predicted from these coordinates are 71F1;72D10. Df(3L)BSC774 failed to complement brm[2], Mbs[3] and Taf4[1].