Isolation and characterization of Df(3L)BSC796. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(3L)BSC796 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC796 was isolated as a FLP recombinase-induced recombination event involving PBacRBmRpL15[e03567] and PXPd06749. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacRBmRpL15[e03567]/PXPd06749 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.RB3BSC796 from the segment of PBacRBmRpL15[e03567] to the left of its FRT site and the segment of PXPd06749 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(3L)BSC796 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:20429215;20723658 and the cytological breakpoints predicted from these coordinates are 77C3;77E4. Df(3L)BSC796 failed to complement kni[ri-1] and Df(3L)BSC448.