Isolation and characterization of Df(2R)BSC813. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(2R)BSC813 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC813 was isolated as a FLP recombinase-induced recombination event involving PXPd02467 and PBacRBCG2921[e02644]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PXPd02467/PBacRBCG2921[e02644] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBacXP5.RB3BSC813 from the segment of PXPd02467 to the left of its FRT site and the segment of PBacRBCG2921[e02644] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(2R)BSC813 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2R:17533641;17963253 and the cytological breakpoints predicted from these coordinates are 57F5;58B3. Df(2R)BSC813 failed to complement Tim10[KG04105], Df(2R)Exel6077 and Df(2R)BSC360.