Isolation and characterization of Df(1)BSC853. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(1)BSC853 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC853 was isolated as a FLP recombinase-induced recombination event involving PBacRBe02417 and PXPd04369. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBacRBe02417/PXPd04369; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}853 from the segment of PBacRBe02417 to the left of its FRT site and the segment of PXPd04369 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(1)BSC853 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are X:17126100;17455865 and the cytological breakpoints predicted from these coordinates are 15F4;16B4. It failed to complement f[1].