Isolation and characterization of Df(3L)BSC839. - Christensen, S.; Cook, K.; Cook, K. (2009)
Isolation and characterization of Df(3L)BSC839 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC839 was isolated as a FLP recombinase-induced recombination event involving PBacWHf03016 and PXPCG5130[d01634]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBacWHf03016/PXPCG5130[d01634] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC839 from the segment of PBacWHf03016 to the left of its FRT site and the segment of PXPCG5130[d01634] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC839 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:20313247;20486308 and the cytological breakpoints predicted from these coordinates are 77B4;77C6. Df(3L)BSC839 failed to complement DNAprim[L5] and fbl[1].