Isolation and characterization of Df(3L)BSC835. - Cook, K.; Christensen, S.; Cook, K. (2009)
Df(3L)BSC835 was isolated as a FLP recombinase-induced recombination event involving PXPd10863 and PBacWHf01995. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPd10863/PBacWHf01995 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP3.WH3BSC835 from the segment of PXPd10863 to the left of its FRT site and the segment of PBacWHf01995 to the right of its FRT site. The breakpoints of Df(3L)BSC835 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:276882;381004 and the cytological breakpoints predicted from these coordinates are 61B3;61C1. Df(3L)BSC835 failed to complement trh[10512] and Df(3L)Exel6084.