Isolation and characterization of Df(2L)BSC860. - Cook, K.; Christensen, S.; Cook, K. (2009)
Df(2L)BSC860 was isolated as a FLP recombinase-induced recombination event involving PBacWHsNPF[f06876] and PXPd05431. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBacWHsNPF[f06876]/PXPd05431 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC860 from the segment of PXPd05431 to the left of its FRT site and the segment of PBacWHsNPF[f06876] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PXPd05431 to be Release 3 genomic coordinate 20280511 on chromosome arm 2L. This corresponds to 38C5 on the Release 3 and Release 5 genome maps. The predicted position of PBacWHsNPF[f06876] on the Release 5 map is 38B1. Consequently, the cytological breakpoints of Df(2L)BSC860 are predicted to be 38B1;38C5. Df(2L)BSC860 failed to complement barr[L305].