Isolation and characterization of Df(3R)BSC887. - Cook, K.; Cook, K. (2010)
Df(3R)BSC887 was isolated as a FLP recombinase-induced recombination event involving PXPCG6006[d09073] and PBacWHCG14894[f04937]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] cu[1] females crossed to P{hsFLP}1, y[1] w[1118]; PXPCG6006[d09073]/PBacWHCG14894[f04937] males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP5.WH5BSC887 from the segment of PXPCG6006[d09073] to the left of its FRT site and the segment of PBacWHCG14894[f04937] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3R)BSC887 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3R:11983756;12268648 and the cytological breakpoints predicted from these coordinates are 89B6;89B16. Df(3R)BSC887 failed to complement Df(3R)Exel7329.