Isolation and characterization of Df(2L)BSC891. - Cook, K.; Cook, K. (2010)
Isolation and characterization of Df(2L)BSC891 Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC891 was isolated as a FLP recombinase-induced recombination event involving PXPescl[d01514] and PBacWHCG31760[f00461]. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y[1] w[1118]; PXPescl[d01514]/PBacWHCG31760[f00461] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBacXP3.WH3BSC891 from the segment of PXPescl[d01514] to the left of its FRT site and the segment of PBacWHCG31760[f00461] to the right of its FRT site. The breakpoints of Df(2L)BSC891 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2L:11990983;12170401 and the cytological breakpoints predicted from these coordinates are 33B5;33D2. Df(2L)BSC891 failed to complement prd[8] and Df(2L)ED775. -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005