UAS-APP and APP-based constructs and insertions from Vitruvean. - Singh, C.; Mahoney, M. (2011)
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Carol Singh and Matt Mahoney of Vitruvean, Inc. PUAS-APP.695.Exel was made by Exelixis, Inc and consists of the 695 amino acid isoform of human APP under the control of UAS in the PExpress-UAS vector (FBtp0012992). P{UAS-APP.695.Exel}5 is a homozygous viable and fertile insertion on the second chromosome. P{UAS-APP.695.Exel}3 is a homozygous viable and fertile insertion on the third chromosome. P{UAS-APP.695.Exel}1 is an insertion on the third chromosome. PUAS-APP.695.E693Q.Exel was made by Exelixis, Inc and consists of the 695 amino acid isoform of human APP carrying the familial Alzheimer's "Dutch" mutation (E693Q - amino acid numbering based on APP sequence) under the control of UAS in the PExpress-UAS vector (FBtp0012992). P{UAS-APP.695.E693Q.Exel}5 is a homozygous viable and fertile insertion on the second chromosome. P{UAS-APP.695.E693Q.Exel}9 is a homozygous viable and fertile insertion on the third chromosome. PUAS-APP.770.E693Q.V715M.myc.VTR consists of the 770 amino acid isoform of human APP carrying the familial Alzheimer's "Dutch" (E693Q) and "French" (V715M) mutations (amino acid numbering based on APP sequence) under the control of UAS in the PUAST vector (FBmc0000383). PUAS-APP.770.E693Q.V715A.myc.VTRconsists of the 770 amino acid isoform of human APP carrying the familial Alzheimer's "Dutch" (E693Q) and "German" (V715A) mutations (amino acid numbering based on APP sequence) under the control of UAS in the PUAST vector (FBmc0000383). PUAS-APP.770.K670N.M671L.VTR consists of the 770 amino acid isoform of human APP carrying the familial Alzheimer's "Swedish" mutation (K670N and M671L - amino acid numbering based on APP sequence) under the control of UAS in the PUAST vector (FBmc0000383). PUAS-APP.770.K670N.M671L.E693G.VTR consists of the 770 amino acid isoform of human APP carrying the familial Alzheimer's "Swedish" (K670N, M671L) and "Arctic" (E693G) mutations (amino acid numbering based on APP sequence) under the control of UAS in the PUAST vector (FBmc0000383). PUAS-Abeta42.B was made by Juan Botas (Baylor College of Medicine) and consists of the 42 amino acid Abeta fragment of human APP under the control of UASin the PUAST vector (FBmc0000383). PUAS-Abeta42.Bm26a is a homozygous viable and fertile insertion on the second chromosome. PUAS-APP.Abeta42.E693G.VTR consists of the 42 amino acid Abeta fragment of human APP carrying the familial Alzheimer's "Arctic" mutation (E693G - amino acid numbering based on APP sequence) under the control of UAS in the PUAST vector (FBmc0000383). P{UAS-APP.Abeta42.E693G.VTR}8 is a homozygous viable and fertile insertion on the third chromosome. PUAS-APP.Abeta42.E693Q.VTR consists of the 42 amino acid Abeta fragment of human APP carrying the familial Alzheimer's "Dutch" mutation (E693Q - amino acid numbering based on APP sequence) under the control of UASin the PUAST vector (FBmc0000383). P{UAS-APP.Abeta42.E693Q.VTR}2 is a homozygous viable and fertile insertion on the second chromosome. P{UAS-APP.Abeta42.E693Q.VTR}3 is an insertion on the third chromosome. PUAS-APP.Abeta42.A692G.VTR consists of the 42 amino acid Abeta fragment of human APP carrying the familial Alzheimer's "Flemish" mutation (A692G - amino acid numbering based on APP sequence) under the control of UASin the PUAST vector (FBmc0000383). P{UAS-APP.Abeta42.A692G.VTR}2 is a homozygous viable and fertile insertion on the third chromosome. PUAS-APP.Abeta42.D694N.VTR consists of the 42 amino acid Abeta fragment of human APP carrying the familial Alzheimer's "Iowa" mutation (D694N - amino acid numbering based on APP sequence) under the control of UASin the PUAST vector (FBmc0000383). P{UAS-APP.Abeta42.D694N.VTR}3 is a homozygous viable and fertile insertion on the third chromosome. PUAS-APP.Abeta42.E693K.VTR consists of the 42 amino acid Abeta fragment of human APP carrying the familial Alzheimer's "Italian" mutation (E693K - amino acid numbering based on APP sequence) under the control of UASin the PUAST vector (FBmc0000383). P{UAS-APP.Abeta42.E693K.VTR}12 is a homozygous viable and fertile insertion on the second chromosome. PUAS-APP.C99.myc.Exel was made by Exelixis, Inc and consists of the C99 fragment of human APP, with the APP signal peptide and a C-terminal myc tag, under the control of UAS in the PExpress-UAS vector (FBtp0012992). P{UAS-APP.C99.myc.Exel}21 is a homozygous viable and fertile insertion on the third chromosome. P{UAS-APP.C99.myc.Exel}19 is a homozygous viable and fertile insertion on the second chromosome. PUAS-APP.C99.Lcp2sp.HA.Exel was made by Exelixis, Inc and consists of the C99 fragment of human APP, with a signal peptide from the fly Lcp2 and a C-terminal tag consisting of three copies of HA, under the control of UAS in the PExpress-UAS vector (FBtp0012992). P{UAS-APP.C99.Lcp2sp.HA.Exel}11 is a homozygous viable and fertile insertion on the third chromosome. PUAS-APP.C99.V717I.HA.Exel was made by Exelixis, Inc and consists of the C99 fragment of human APP carrying the familial Alzheimer's "London" mutation (V717I -amino acid numbering based on APP sequence), with the human APP signal peptide and a C-terminal tag consisting of three copies of HA, under the control of UAS in the PExpress-UAS vector (FBtp0012992). P{UAS-APP.C99.V717I.HA.Exel}9 is a homozygous viable and fertile insertion on the third chromosome. PUAS-APP.C99.YIPLFL.HA.Exel was made by Exelixis, Inc and consists of the C99 fragment of human APP carrying amino acids YIPLFL instead of GVVIAT (APP amino acids 709-714), with the human APP signal peptide and a C-terminal tag consisting of three copies of HA, under the control of UAS in the PExpress-UAS vector (FBtp0012992). P{UAS-APP.C99.YIPLFL.HA.Exel}3 is a homozygous viable and fertile insertion on the third chromosome. P{UAS-APP.C99.YIPLFL.HA.Exel}4a is a homozygous viable insertion on the second chromosome.