Spokony insertions. - Spokony, R.; White, K. (2012)
The following information and information in a linked spreadsheet accompanied stocks donated to the Bloomington Stock Center by Rebecca Spokony and Kevin White, University of Chicago. BAC clones were recombineered to introduce sequences encoding fluorescence or epitope tags at specific positions within genes to tag specific protein isoforms. The clones were integrated at specific attP sites by phiC31 integrase-mediated transformation. Injections we done in the lab of Thom Kaufman at Indiana University by Stacy Holtzman. Though the constructs are named for the tagged gene, other genes are often present in the constructs. In the construct names, 'GFP.FPTB', 'GFP.FSTF' and 'FTSF.GFP' represent combinations of epitope tags and peptide sequences: GFP.FPTB is a C-terminal superfolderGFP-FLAG-PreScission-TEV-BLRP tag combination. GFP.FSTF is a C-terminal EGFP-FlAsH-StrepII-TEV-3xFlag tag combination. FTSF.GFP is an N-terminal 3xFlag-TEV-StrepII-EGFP-FlAsH tag combination. Information about the protein isoforms tagged by each construct is included in the downloadable spreadsheet. The following insertions are described in the spreadsheet. PBacAbd-B-EGFP.SVK00037 PBacab-GFP.FLAGVK00033 PBacAtf-2-GFP.FLAGVK00033 PBacbab2-GFP.FPTBVK00037 PBacbrk-GFP.FPTBVK00033 PBacbr-GFP.FPTBVK00033 PBaccnc-EGFP.SVK00037 PBacCHES-1-like-EGFP.SVK00033 PBacdm-GFP.FPTBVK00033 PBacdisco-r-EGFP.SVK00033 PBacdl-GFP.FLAGVK00033 PBacEip74EF-GFP.FLAGVK00037 PBacEip78C-GFP.FLAGVK00037 PBacERR-GFP.FSTFVK00037 PBacEts21C-GFP.FLAGVK00033 PBacEts65A-GFP.FLAGVK00037 PBacEts97D-GFP.FLAGVK00037 PBacexd-EGFP.SVK00033 PBaceyg-GFP.FPTBVK00037 PBacfoxo-GFP.FLAGVK00037 PBacftz-f1-GFP.FLAGVK00037 PBacgcm2-GFP.FLAGVK00033 PBacgcm-GFP.FLAGVK00033 Pgro-GFP.FLAGattP16 PBacHnf4-GFP.FLAGVK00033 PBacHr51-GFP.FLAGVK00033 PBacHr38-GFP.FLAGVK00033 PBacHr39-GFP.FPTBVK00033 PBacHr78-GFP.FLAGVK00037 PBacFTSF.GFP-Hr96VK00037 PBacHr4-GFP.FSTFVK00033 PBacjing-GFP.FPTBVK00033 PBackay-GFP.FLAGVK00037 PBackn-GFP.FPTBVK00033 PBaclola.BC-GFP.FLAGVK00033 PBaclola.J-GFP.FLAGVK00033 PBaclola.GR-GFP.FLAGVK00033 PBaclola.I-GFP.FLAGVK00033 PBacMad-GFP.FLAGVK00033 PBacnmo-GFP.FLAGVK00037 PBacN-GFP.FLAGVK00033 PBacFTSF.GFP-sensVK00022 PBacsvp-GFP.FLAGVK00037 PBacslou-EGFP.SVK00037 PBacSp1-EGFP.SVK00033 PBacStat92E-GFP.FLAGVK00037 PBactio-GFP.FPTBVK00033 PBacusp-GFP.FLAGVK00033 PBacvri-GFP.FPTBVK00033 PBaczfh2-GFP.FPTBVK00033