shtd constructs and insertions. - Matakatsu, M. (2014)
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Miho Matakatsu, University of Chicago. Pshtd.T7 is a rescue construct consisting of a 9518 bp SalI-SpeI fragment containing 1822 bp of shtd 5' enhancer/promoter region. shtd is tagged with T7 at the C-terminus. P{shtd.T7}2 is a homozygous viable and fertile insertion on the second chromosome. PUAS-HA.shtd expresses full length shtd tagged with HA at the N-terminus under the control of UAS. P{UAS-HA.shtd}2 is a homozygous viable insertion on the second chromosome. PUAS-shtd.T7 expresses full length shtd tagged with T7 at the C-terminus under the control of UAS. P{UAS-shtd.T7}2 is a homozygous viable and fertile insertion on the second chromosome.