Genomic BAC constructs containing epitope tagged proteins. - Victorsen, A.; White, K. (2014)
The following information and information in a linked spreadsheet accompanied stocks donated to the Bloomington Stock Center by Alec Victorsen and Kevin White, University of Chicago. BAC clones were recombineered to introduce sequences encoding fluorescence or epitope tags at specific positions within genes to tag specific protein isoforms. The clones were integrated at specific attP sites by phiC31 integrase-mediated transformation. Though the constructs are named for the tagged gene, other genes are often present in the constructs. In the construct names, 'GFP.FPTB' represents combinations of epitope tags and peptide sequences: GFP.FPTB is a C-terminal superfolderGFP-FLAG-PreScission-TEV-BLRP tag combination. Information about the protein isoforms tagged by each construct is included in the downloadable spreadsheet. The following insertions are described in the spreadsheet. PBacDip3-GFP.FPTBVK00031 PBacfd3F-GFP.FPTBVK00033 PBacCG8388-GFP.FPTBVK00033