Watnick constructs and insertions. - Watnick, P. (2015)
The following information accompanied stocks donated to the Bloomington Stock Center by Paula Watnick, Boston Children's Hospital. PUAS-mtd.RD is a construct expressing the coding region of the "RD" transcript (according to FB2010_07, released September 3rd, 2010) from the mtd gene under UAS control. The coding region was amplified from cDNA using primers that placed a NotI site immediately adjacent to the ATG start codon and an EcoRI site immediately adjacent to the TGA stop codon. The amplified fragment was cloned into the polylinker of pPUAST following NotI and EcoRI digestion. PUAS-mtd.RB is a pPUAST construct expressing the protein encoded by the mtd RB transcript under UAS control. PUAS-OXR1.HA is a pPUAST construct expressing human OXR1 protein with a C-terminal HA tag under UAS control. PUAS-NCOA7.HA is a pPUAST construct expressing human NCOA7 protein with a C-terminal HA tag under UAS control. PUAS-TLDC2.HA is a pPUAST construct expressing human TLDC2 protein with a C-terminal HA tag under UAS control. PUAS-CG17244.HA is a pPUAST construct expressing CG17244 protein with a C-terminal HA tag under UAS control. PCG17244-GAL4 expresses GAL4 under control of the CG17244 regulatory sequences within 1 kb of the transcription start site. P{UAS-mtd.RC}6, P{UAS-mtd.RH}1, P{UAS-mtd.RD}8, P{UAS-mtd.RB}9, P{UAS-OXR1.HA}1, P{UAS-NCOA7.HA}1, P{UAS-TLDC2.HA}2, P{UAS-CG17244.HA}2 and P{CG17244-GAL4}5 are second chromosome insertions. P{UAS-OXR1.HA}4, P{UAS-NCOA7.HA}2, P{UAS-CG17244.HA}1 and P{CG17244-GAL4}1 are third chromosome insertions. -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center http://flystocks.bio.indiana.edu