Carroll constructs and insertions. - Trautman, J.; Carroll, D. (2016)
The following information accompanied stocks donated to the Bloomington Stock Center by Jonathan Trautman and Dana Carroll, University of Utah. PUAS-ryA expresses the same zinc finger nuclease directed against the ry gene that is encoded by Phs-ryA (FBtp0021952) under UAS control. PUAS-ryB expresses the same zinc finger nuclease directed against the ry gene that is encoded by Phs-ryB (FBtp0021953) under UAS control. Phs-yA.P501T.I538K expresses the same zinc finger nuclease encoded by Phs-yA (FBtp0016058) except for substitution of sequences encoding Threonine for Proline at residue 501 and Lysine for Isoleucine at residue 538. The P501T substitution corrects a missense mutation to give the wild type FokI sequence and the I538K substitution prevents homodimerization of the zinc finger nuclease. The construct was generated in a Casper-based vector. Phs-yB.Q486E.P501T expresses the same zinc finger nuclease encoded by Phs-yB (FBtp0016057) except for substitution of sequences encoding Threonine for Proline at residue 501 and Glutamic acid for Glutamine for at residue 486. As above, the P501T substitution corrects a missense mutation to give the wild type FokI sequence. The Q486E substitution prevents homodimerization of the zinc finger nuclease. The construct was generated in a Casper-based vector. P{hs-ryA}1C, P{hs-yA}20b, P{hs-yB}7a, P{hs-yA.P501T.I538K}2C, P{UAS-ryA}14C and P{UAS-ryB}1B are second chromosome insertions. P{y[M.donor]}3, P{hs-ryB}2C and P{hs-yB.Q486E.P501T}2C are third chromosome insertions. -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center http://flystocks.bio.indiana.edu