PR52B07-GAL4.DBD, PR87B09-GAL4.DBD and PBacR52H01-p65.AD constructs and insertions. - Rubin, G.M. (2017)
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Gerald M. Rubin, Howard Hughes Medical Institute, Janelia Research Campus. PR52B07-GAL4.DBD is composed of the GMR52B07 enhancer fragment fused upstream of the Drosophila synthetic core promoter (DSCP) followed by a leucine zipper (T:Zzzz\Zip-) fused to the Scer\GAL4 DNA-binding domain (which has been optimised for Drosophila codon usage). A Hsp70 transcriptional terminator is present. The MB085C line in Aso et al 2014 (FBrf0227179) is composed of PBacR52H01-p65.ADVK00027 and PR52B07-GAL4.DBDattP2. PR52B07-GAL4.DBDattP2 is an insertion on the third chromosome in an attP site at 68A4, 3L:11063638. PR87B09-GAL4.DBD is composed of the GMR87B09 enhancer fragment fused upstream of the Drosophila synthetic core promoter (DSCP) followed by a leucine zipper (T:Zzzz\Zip-) fused to the Scer\GAL4 DNA-binding domain (which has been optimised for Drosophila codon usage). A Hsp70 transcriptional terminator is present. The MB045B line is composed of PR58E02-p65.ADattP40 and PR87B09-GAL4.DBDattP2. PR87B09-GAL4.DBDattP2 is an insertion on the third chromosome in an attP site at 68A4, 3L:11063638. PBacR52H01-p65.AD is similar to PR52H01-p65.AD (FBtp0099408). PBacR52H01-p65.ADVK00027 is an insertion on the third chromosome in an attP site at 89E11, 3R:17052863.