RNAi resistant polo constructs and insertions. - Archambault, V. (2019)
The following information accompanied stocks donated to the Bloomington Stock Center by Vince Archambault, Universite de Montreal. PUASp-polo-T182A.NLS7A.GFP.RNAi-res is identical to PUASp-polo.GFP.RNAi-res except for sequence changes that substitute seven alanines into the nuclear localization sequence and a non-phosphorylatable alanine for threonine 182. PUASp-polo.T182D.NLS7A.GFP.RNAi-res is identical to PUASp-polo.GFP.RNAi-res except for sequence changes that substitute seven alanines into the nuclear localization sequence and a phosphomimetic aspartic acid for threonine 182. Both constructs were generated in work related to that presented in Karchaner et al. (2017; FBrf0237276). PUASp-polo.T182D.NLS7A.GFP.RNAi-resattP154 and PUASp-polo-T182A.NLS7A.GFP.RNAi-resattP154 are insertions into PCaryPattP154 at 97D1, 3R:26798991 (R6).