Location data for daed mutations. - Czech, B. (2020)
First, we have noticed that in our stock used to make the CRISPR mutants (BL 51323), the daed gene actually carries a mutation in the start codon, which leads to a 7aa N-terminally truncated protein. It might be worth highlighting this or perhaps even adding this as a variant. The daed[?2*] mutant is a deletion that removes the new start codon 21nt downstream of the annotated one and results in a putative truncated protein that would lack the SAM domain. The sequences flanking the deletion are as follows: daed[?2] upstream TAATTTAATTGCCATTATTGACAGAGTTTAaGGCAACGAAGACGCACACA daed[?2] downstream CATTCAGTTTGAGGCCACAAAGAACTTTCACTCGCACACCGAGCAGAAGG For daed[oof1] the situation is a little more complex, as there are some base substitutions in addition to the deletion that cause this mutant to go out of frame very near the start codon. daed[oof1] upstream TTAATTGCCATTATTGACAGAGTTTAaGGCAACGAAGACGCACACAATGT daed[oof1] downstream ATTCAGTTTGAGGCCACAAAGAACTTTCACTCGCACACCGAGCAGAAGGA With the following bases left in this mutant between the above sequences: ttCa