NIG-Fly sgRNA and KO collections. - National Institute of Genetics Fly Stocks (2022)
This personal communication describes two collections of stocks available from the fly stock collection of the National Institute of Genetics (NIG), Mishima, Japan. The NIG-Fly sgRNA collection consists of fly stocks containing transgenic constructs designed for CRISPR/Cas9-based mutation of a gene. Each construct constitutively expresses a single sgRNA that targets a gene of interest. When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of this target gene is induced, resulting in an indel (small insertion or deletion mutation). The NIG-Fly KO collection consists of fly stocks, each carrying a knockout of a gene of interest, generated using stocks from the NIG-Fly sgRNA collection. The associated files for this personal communication correspond to the downloadable files available from the NIG-Fly website for each collection: NIG-Fly sgRNA collection: Downloaded from https://shigen.nig.ac.jp/fly/nigfly/download/files/grna.tsv Date last updated: 26.9.2022 NIG-Fly KO collection: Downloaded from https://shigen.nig.ac.jp/fly/nigfly/download/files/ko.tsv Date last updated: 26.9.2022