D. Cai constructs and insertions. - Cai, D. (2023)
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Dawen Cai, University of Michigan, Ann Arbor: PBac{nSyb(FRT13.FLP)} Self-regulating Flippase under the regulation of the n-Synaptobrevin promoter. FLP sequence is flanked by two FRT-F13 sites, corresponding to medium excising probability hence medium FLP activity. P{nSyb(FRT15.FLP)} Self-regulating Flippase under the regulation of the n-Synaptobrevin promoter. FLP sequence is flanked by two FRT-F15 sites, corresponding to low excising probability hence longer FLP activity. PUAS-nBitbow.1.0su(Hw)attP5 is an insertion at the PCaryIPsu(Hw)attP5 site. PUAS-gBitbow.1.0su(Hw)attP5 is an insertion at the PCaryIPsu(Hw)attP5 site. PBacUAS-gBitbow.1.0VK00027 is an insertion at the PBac{y+-attP-9A}VK00027 site. PUAS-mBitbow.1.0su(Hw)attP5 is an insertion at the PCaryIPsu(Hw)attP5 site. PBacUAS-mBitbow.1.0VK00027 is an insertion at the PBac{y+-attP-9A}VK00027 site. PBac{nSyb(FRT13.FLP)}VK00027 is an insertion at the PBac{y+-attP-9A}VK00027 site. P{nSyb(FRT15.FLP)}attP2 is an insertion at the PCaryPattP2 site. PUAS-hH2B.2xmNGattP2 is an insertion at the PCaryPattP2 site. PBacUAS-hH2B.2xmNGVK00027 is an insertion at the PBac{y+-attP-9A}VK00027 site. PUAS-hH2B.2xTagBFPattP2 is an insertion at the PCaryPattP2 site. PBacUAS-hH2B.2xTagBFPVK00027 is an insertion at the PBac{y+-attP-9A}VK00027 site.