A set of ~50 targeted deficiencies created by exploiting hybrid element insertion (HEI) and resolution; designed to fill gaps in deletion coverage.
30C3-30C5;30F1
The 2L:9 ,706,076 release 6 coordinate of the left breakpoint is an estimate. It corresponds to the insertion site of P{PZ}pelo1. As FBrf0175003 explains, deletions generated by P transposase in the presence of trans-heterozygous P elements are expected to extend from the end of one P insertion to the vicinity of the other P insertion. The absence of miniwhite from the Df(2L)BSC17 chromosome suggests that the deletion extends from the right end of the P{PZ}pelo1 insertion to the vicinity of P{EP}DrefEP460.
The 2L:9967229 release 6 coordinate of the right breakpoint is an estimate. It corresponds to the insertion site of P{EP}DrefEP460. As FBrf0175003 explains, deletions generated by P transposase in the presence of trans-heterozygous P elements are expected to extend from the end of one P insertion to the vicinity of the other P insertion. The absence of miniwhite from the Df(2L)BSC17 chromosome suggests that the deletion extends from the right end of the P{PZ}pelo1 insertion to the vicinity of P{EP}DrefEP460. Heterozygosity for Df(2L)BSC17 does not cause Minute phenotypes, so the right breakpoint must lie to the left of the haploinsufficient RpL13 gene.
Flies heterozygous for the deletion do not show a Minute bristle phenotype.
The miniwhite marker from P{EP}DrefEP460 was deleted or disrupted.